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While Drosophila ELAV is required as far as something the correct differentiation and endurance of neurons, the roles played by the Hu genes dressed in the mammalian nervous system stay put largely unknown.
To explore the in vivo functions of mouse Hu proteins, we overexpressed them in rat pheochromocytoma PC12 cells, where they induced neuronal phenotype in the absence of the whim-whams growth factor. We have characterized the functions of various forms of mHuB and mHuC behaviour point mutations or deletions.
Concerning addition, the mutants containing no more than the third RRM failed en route for induce the neuronal phenotype at home PC12 cells and inhibited the biologic activity of cotransfected wild-type mHuB and mHuC, thus drama as a dominant-negative form. Anyhow, these mutants could not terminate the nerve growth factor-induced segregation of PC12 cells. Further, we misexpressed wild-type and dominant-negative Hu in E9. Christian ponder dating espn reporter car.
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- This work was supported by the Comité Interprofessionnel du Vin de Bordeaux ( CIVB) (predoctoral...
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- A Nuclear Function of Hu Proteins as Neuron-specific Alternative RNA Processing Regulators
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